Thermostable Enzyme Laboratory Product

Enzyme

Oxidoreductases (Reaction ^ oxidation and reduction)

Laccase YK1

Volume^2mlA1ml
EC No.^1.10.3.2
Substrate^ benzenndiol
Reaction product^ benzosemiquinone
Reaction temperature ^ `95Ž
Reaction pH ^ 3.0`5.0

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Alcohol dehydrogenase YK1

Volume^1mlA0.5ml
EC No.^1.1.1.1
Substrate^ enanthaldehyde (acetaldehyde)
Reaction product^ 1-heptanol (alcohol)
Reaction temperature ^ `85Ž
Reaction pH ^ 4.5`7.0

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Alcohol dehydrogenase HI1

Volume^1mlA0.5ml
EC No.^1.1.1.1
Substrate^ propionaldehyde (aldehyde)
Reaction product^ 1-propanol (alcohol)
Reaction temperature ^ `65Ž
Reaction pH ^ 4.5`7.0

–Used in Enzymatic Ethanol Production Kit

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Alcohol dehydrogenase HI2

Volume^1mlA0.5ml
EC No.^1.1.1.1
Substrate^ butylaldehyde (aldehyde)
Reaction product^ 1-butanol (alcohol)
Reaction temperature ^ |
Reaction pH ^ 4.5`6.5

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Secondary alcohol dehydrogenase A

Volume^1mlA0.5ml
EC No.^1.1.1.1
Substrate^ 1,2-propanediol(secondary alcohol)
Reaction product^ hydroxyacetone(ketone)
Reaction temperature ^ `70Ž
Reaction pH ^ 8.5`10.5

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Secondary alcohol dehydrogenase B

Volume^5mlA2.5ml
EC No.^1.1.1.1
Substrate^ 1,2-propanediol(secondary alcohol)
Reaction product^ hydroxyacetone(ketone)
Reaction temperature ^ `85Ž
Reaction pH ^ 8.0`10.0

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6-Phosphogluconate dehydrogenase

Volume^1ml
EC No.^1.1.1.44
Substrate^ 6-phosphogluconate
Reaction product^ ribulose 5-phosphate + CO2
Reaction temperature ^ `70Ž
Reaction pH ^ 6.0`9.0

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Glyceraldehyde-3-phosphate dehydrogenase YK1

Volume^1ml
EC No.^1.2.1.12
Substrate^ glyceraldehyde-3-phosphate
Reaction product ^ 1,3-bisphosphoglyceric acid
Reaction temperature ^ `70Ž
Reaction pH ^ 8.0`11.0

–Used in Enzymatic Ethanol Production Kit

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Oxidoreductases-2 (Reaction ^ oxidation and reduction)

Glycerol dehydrogenase HI1

Volume^0.5ml
EC No.^1.1.1.6
Substrate^ 1,2-propandiol(or glycerol)
Reaction product^ hydroxiacetone (or dihydroxyacetone)
Reaction temperature ^ `100Ž
Reaction pH ^ 7.0`10.0

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Glucose 1-dehydrogenase

Volume^1ml
EC No.^1.1.1.47
Substrate^ glucose
Reaction product^ gluconolactone
Reaction temperature ^ `70Ž
Reaction pH ^ 5.0`8.0

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Glucose-6-phosphate dehydrogenase

Volume^1ml
EC No.^1.1.1.49
Substrate^ glucose-6-phosphate
Reaction product^ 6-phosphogluconate
Reaction temperature ^ `70Ž
Reaction pH ^ 6.5`8.0

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Lactate dehydrogenase 2

Volume^1.5mlA0.5ml
EC No.^1.1.1.27
Substrate^
Reaction product^
Reaction temperature ^ `70Ž
Reaction pH ^ 6.0`8.5

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Hydrolases (Reaction ^ hydrolysis)

Chitinase

Volume^1ml
EC No.^3.2.1.14
Substrate^ chitin
Reaction product ^chitobiose
Reaction temperature ^ |
Reaction pH ^ 3.0`8.0

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ƒÀ-1,3-Glucanase-A

Volume^1ml
EC No.^3.2.1.39
Substrate^ ƒÀ-1,3-glucan
Reaction product ^ ƒÀ-1,3-glucan oligomer
Reaction temperature ^ `85Ž
Reaction pH ^ 4.0`6.5

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ƒÀ-1,3-Glucanase-B

Volume^1ml
EC No.^3.2.1.39
Substrate^ ƒÀ-1,3-glucan
Reaction product ^ ƒÀ-1,3-glucan oligomer
Reaction temperature ^ `90Ž
Reaction pH ^ 4.0`8.0

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ƒÀ-1,3-Glucanase-C

Volume^1ml
EC No.^3.2.1.39
Substrate^ ƒÀ-1,3-glucan
Reaction product ^ ƒÀ-1,3-glucan oligomer
Reaction temperature ^ `50Ž
Reaction pH ^ 4.5`7.5

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Cellulase Cel.1

Volume^50mlA25ml
EC No.^3.2.1.4
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product ^ cellobiose
Reaction temperature ^ |
Reaction pH ^ 4.5`7.0

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Cellulase HT1

Volume^10mlA5ml
EC No.^3.2.1.4
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product ^ cellobiose
Reaction temperature ^ `75Ž
Reaction pH ^ 4.5`8.5

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Cellulase HT2

Volume^10mlA5ml
EC No.^3.2.1.4
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product ^ cellobiose
Reaction temperature ^ `80Ž
Reaction pH ^ 5.0`7.0

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Cellulase HT3

Volume^10mlA5ml
EC No.^3.2.1.4
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product ^ cellobiose
Reaction temperature ^ `75Ž
Reaction pH ^ 5.5`7.0

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Cellulase HT4

Volume^10mlA5ml
EC No.^3.2.1.4
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product^ cellobiose
Reaction temperature ^ `80Ž
Reaction pH ^ 4.0`6.0

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Hydrolases-2 (Reaction ^ hydrolysis)

Pullulanase KS1

Volume^10mlA5ml
EC No.^3.2.1.41
Substrate^ pullulan
Reaction product ^ maltotriose
Reaction temperature ^ `90Ž
Reaction pH ^ 4.5`7.0

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ƒÀ-Glucosidase HT1

Volume^1ml
EC No.^3.2.1.74
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product ^ glucose
Reaction temperature ^ `65Ž
Reaction pH ^ 4.0`6.5

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Cellobiohydrolase HT1

Volume^10mlA5ml
EC No.^3.2.1.91
Substrate^ ƒÀ-1,4-glucan (cellulose)
Reaction product ^ cellobiose
Reaction temperature ^ `80Ž
Reaction pH ^ 5.0`7.0

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ƒÀ-Xylosidase YK1

Volume^1ml
EC No.^3.2.1.37
Substrate^ xylo-oligosaccharides
Reaction product^ xylose
Reaction temperature ^ `100Ž
Reaction pH ^ 4.0`8.0

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ƒÀ-Mannanase TT1

Volume^10ml
EC No.^3.2.1.78
Substrate^ mannan
Reaction product^ mannno-origosaccaharidesAmannose
Reaction temperature ^ `100Ž
Reaction pH ^ 4.5`7.0

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Amyloglucosidase

Volume^10mlA5ml
EC No.^3.2.1.3
Substrate^ƒ¿-1,4-glucan
Reaction product ^glucose
Reaction temperature ^ `80Ž
Reaction pH ^ 4.0`5.0

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Kinases (Reaction ^ phosphorylation)

Glucokinase YK1

Volume^1ml
EC No.^2.7.1.2
Substrate^ glucose
Reaction product ^ glucose-6-phosphate
Reaction temperature ^ `80Ž
Reaction pH ^ 7.5`11.0

–Used in Enzymatic Ethanol Production Kit

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Pyruvate kinase HI1

Volume^1ml
EC No.^2.7.1.40
Substrate^ phosphoenolpyruvic acid
Reaction product ^ pyruvic acid
Reaction temperature ^ `75Ž
Reaction pH ^ 5.0`8.0

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Pyruvate kinase KS1

Volume^1ml
EC No.^2.7.1.40
Substrate^ phosphoenolpyruvic acid
Reaction product ^ pyruvic acid
Reaction temperature ^ `85Ž
Reaction pH ^ 5.0`8.0

–Used in Enzymatic Ethanol Production Kit

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Lyases (Reaction ^ cleaving various bonds)

Pyruvate decarboxylase YK1

Volume^0.2ml
EC No.^4.1.1.1
Substrate^ pyruvic acid
Reaction product ^ acetaldehyde
Reaction temperature ^ `50Ž
Reaction pH ^ 5.0`7.5

–Used in Enzymatic Ethanol Production Kit

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Isomerases (Reaction ^ isomerization)

Xylose isomerase A (Glucose isomerase A)

Volume^10mlA5ml
EC No.^5.3.1.5
Substrate^ xylose
Reaction product ^ xylulose
Reaction temperature ^ |
Reaction pH ^ 6.0`8.0

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Others

Expansin

Volume^1mlA10ml
EC No.^|
Substrate^|
Reaction product^|
Reaction temperature ^ |
Reaction pH ^ |

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Enzymatic Ethanol Production KitF
12 kinds of enzymes take part in conversion from the glucose to the ethanol. This kit is an experimental kit to enable conversion from the glucose to the ethanol by the continuous enzyme reaction with those 12 kinds of thermostable enzymes in vitro.

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Made-to-order enzyme

We manufacture enzymes of your choice upon request.

Transferases(Reaction / transferring a functional group)

Deoxy ribose-phosphate aldolase
EC No.^4.1.2.4
Substrate^ 2-deoxy-D-ribose 5-phosphate
Reaction product ^ D-glyceraldehyde-3-phosphate + acetaldehyde
Reaction temperature ^ |
Reaction pH ^ |

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Transferases(Reaction / transferring a functional group)

Transketolase
EC No.^2.2.1.1
Substrate^ ribose 5-phosphate + xylulose 5-phosphate
Reaction product ^ sedoheptulose 7-phosphate + glyceraldehyde-3-phosphate

Substrate^ erythrose 4-phosphate + xylulose 5-phosphate
Reaction product^ fructose-6-phosphate + glyceraldehyde-3-phosphate

Reaction temperature ^ |
Reaction pH ^ 7.0`9.0

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HydrolasesiReaction / hydrolysis j

Fructose-1,6-bisphosphatase

EC No.^3.1.3.11
Substrate^ fructose-1,6-bisphoaphate
Reaction product^ fructose-6-phosphate
Reaction temperature ^ `65Ž
Reaction pH ^ 7.0

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HydrolasesiReaction / oxidation and Reduction j

Aldehyde dehydrogenase

EC No.^1.2.1.3
Substrate^ aldehyde
Reaction product^ carboxylate
Reaction temperature ^ `55Ž
Reaction pH ^ 6.0`9.5

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HydrolasesiReaction / oxidation and Reduction j

Nitrite reductase

EC No.^1.7.2.1
Substrate^ nitrite
Reaction product^ nitric oxide
Reaction temperature ^ `80Ž
Reaction pH ^ 6.0`7.0

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HydrolasesiReaction / oxidation and Reduction(NADH) j

Glutamate dehydrogenase

EC No.^1.4.1.2
Substrate^ ƒ¿-ketoglutarate + ammonium
Reaction product^ glutamate
Reaction temperature ^ `80Ž
Reaction pH ^ 7.0`9.5

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HydrolasesiReaction / oxidation and Reduction j

Alditol oxidase

EC No.^1.1.3.41
Substrate^ sorbitol(or xylitol)
Reaction product^ glucose(or xylose)
Reaction temperature ^ `75Ž
Reaction pH ^ 5.5`9.5

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HydrolasesiReaction / oxidation and Reduction j

Urate oxidase

EC No.^1.7.3.3
Substrate^ urate
Reaction product^ 5-Hydroxyisourate
Reaction temperature ^ `55Ž
Reaction pH ^ 6.5`10.5

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HydrolasesiReaction / oxidation and Reduction(NADPH) j

Grycerol-3-phosphate dehydrogenase A

EC No.^1.1.1.94
Substrate^ dihydroxyacetone phosphate
Reaction product^ glycerol-3-phosphate
Reaction temperature ^ `75Ž
Reaction pH ^ 6.5`9.0

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HydrolasesiReaction / oxidation and Reduction j

Grycerol-3-phosphate dehydrogenase B

EC No.^1.1.5.3
Substrate^ glycerol-3-phosphate
Reaction product^ dihydroxyacetone phosphate
Reaction temperature ^ `70Ž
Reaction pH ^ 6.0`9.5

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Made-to-order enzyme2

TransferasesiReaction / phosphorylationj

Acetate kinase

EC No.^2.7.2.1
Substrate^ acetyl phosphate + ADP
Reaction product^ acetate + ATP
Reaction temperature ^ `80Ž
Reaction pH ^ 6.0`7.5

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KinasesiReaction / phosphorylationj

Polyphosphate kinase

EC No.^2.7.4.1
Substrate^ polyphosphate(n) + ADP
Reaction product^ polyphosphate(n-1) + ATP
Reaction temperature ^ `80Ž
Reaction pH ^ 4.5`5.5

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KinasesiReaction / phosphorylationj

Adehylate kinase

EC No.^2.7.4.3
Substrate^ 2ADP
Reaction product^ ATP + AMP
Reaction temperature ^ `90Ž
Reaction pH ^ 4.5`6.0

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LyasesiReaction / addition(aldol condensation) j

KDG aldolase

EC No.^4.1.2.20
Substrate^ pyruvate + aldehyde
Reaction product^ aldol condensation product
Reaction temperature ^ `85Ž
Reaction pH ^ 4.0`7.0

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LyasesiReaction / dehydration reactionj

Enolase YK1

EC No.^4.2.1.11
Substrate^ 2-phosphoglycerate
Reaction product^ phosphoenolpyruvic acid
Reaction temperature ^ `80Ž
Reaction pH ^ 5.5`7.5

–Used in Enzymatic Ethanol Production Kit

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LyasesiReaction / aldol cleavage reacitonj

Fructose-1,6-bisphosphate aldolase HI1

EC No.^4.1.2.13
Substrate^ fructose-1,6-bisphosphate
Reaction product^ dihydroxyacetone phosphate + glyceraldehyde-3-phosphate
Reaction temperature ^ `100Ž
Reaction pH ^ 5.0`8.0

–Used in Enzymatic Ethanol Production Kit

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IsomerasesiReaction / isomerizationj

Glucosephosphate isomerase YK1

EC No.^5.3.1.9
Substrate^ glucose-6-phosphate
Reaction product^ fructose-6-phosphate
Reaction temperature ^ |
Reaction pH ^ 6.0`9.0

–Used in Enzymatic Ethanol Production Kit

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Oxide reductasesiReaction / oxidation and reduction (NAD+ or NADP+)j

Malate dehydrogenase

EC No.^1.1.1.37
Substrate^ malic acid
Reaction product^ oxaloacetic acid
Reaction temperature ^ `85Ž
Reaction pH ^ 9.0`12.0

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Oxide reductasesiReaction / decarboxylation (NAD+)j

Malate dehydrogenase(Decarboxylating)

EC No.^1.1.1.38
Substrate^ malic acid or oxaloacetic acid
Reaction product^ pyruvic acid + CO2
Reaction temperature ^ `70Ž
Reaction pH ^ 7.0`10.5

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KinasesiReaction / phosphorylationj

Phosphofructokinase

EC No.^ 2.7.1.11
Substrate^ fructose-6-phosphate
Reaction product^ fructose-1,6-bisphosphate
Reaction temperature ^ `60Ž
Reaction pH ^ 9.0`11.0

–Used in Enzymatic Ethanol Production Kit

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Made-to-order enzyme3

KinasesiReaction / phosphorylationj

Phosphoglycerate kinase YK1

EC No.^2.7.2.3
Substrate^ 3-phosphoglycerate
Reaction product^ 1,3-bisphosphoglycerate
Reaction temperature ^ `85Ž
Reaction pH ^ 8.0`11.0

–Used in Enzymatic Ethanol Production Kit

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IsomerasesiReaction / isomerizationj

Phosphoglycerate mutase HI1

EC No.^5.4.2.1
Substrate^ 3-phosphoglycerate
Reaction product^ 2-phosphoglycerate
Reaction temperature ^ `75Ž
Reaction pH ^ 6.0`9.5

–Used in Enzymatic Ethanol Production Kit

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IsomerasesiReaction / isomerizationj

Triosephosphate isomerase YK1

EC No.^5.3.1.1
Substrate^ dihydroxyacetone phosphate
Reaction product^ glyceraldehyde-3-phosphate
Reaction temperature ^ `95Ž
Reaction pH ^ 5.0`8.0

–Used in Enzymatic Ethanol Production Kit

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KinasesiReaction / phosphorylationj

Xylulokinase A

EC No.^2.7.1.17
Substrate^ xylulose
Reaction product^ xylulose 5-phosphate
Reaction temperature ^ `60Ž
Reaction pH ^ 7.0`9.0

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KinasesiReaction / phosphorylationj

Xylulokinase B

EC No.^2.7.1.17
Substrate^ xylulose
Reaction product^ xylulose 5-phosphate
Reaction temperature ^ `60Ž
Reaction pH ^ 7.0`9.0

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Oxide reductasesiReaction / oxidation and reductionj

Bilirubin oxidase

EC No.^1.3.3.5
Substrate^ bilirubin
Reaction product^ biliverdin + H2O
Reaction temperature ^ `70Ž
Reaction pH ^ 3.0`6.0

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Thermostable Enzyme Laboratory Co., Ltd.
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TEL@i078j302-5502
Email@info@tainetsu.com